sing Enzymes to Make Juice
Partner:
Objective: To determine the best enzyme juice extracting mixture from an apple.
Hypothesis: A mixture of 50% cellulose and 50% pectinase will extract more juice than using either one by itself.
Background Info:
- A catalyst speeds up chemical reaction rates without itself being changed.
- Enzymes are special proteins that work as catalysts which promote chemical reactions in living things.
- Enzymes work by either making molecules or breaking them.
- Enzymes usually end with the root ase, such as in pectinase and cellulase.
- Plant support (to stand upright) is due to cellulose in plant cell walls and pectin found in plant cell walls and between
cells in the Middle lamella.
- Cellulase breaks down cellulase and pectinase breaks down pectin.
- To make juice is a two step process: 1. peeling to remove peel & seeds, and juicing to extract up to 20% more juice
from pulp.
- These enzymes come from the fungi Aspergillus niger.
Materials:
plastic wrap acetate water apples
beaker thermometer cellulase funnel
weight boat pectinase graduated cylinder wood sticks
Methods: 1. 50g of small pieces of apple was weighed out in a weight boat.
2. The apple pieces were transerred to the buffer (control) beaker.
3. Steps 1 & 2 were repeated for pectinase, cellulase, and the "both" beakers.
4. 5mL of acetate buffer was measured and added to the buffer (control beaker).
5. The cylinder was rinsed out and step 4 was repeated for the pectinase and cellulase enzyme samples.
6. The pectinase/cellulase sample was added to the "both" beaker.
7. The beakers were covered with plastic wrap and set in a 45°C bath water for 20 min.
8. Separate wooden sticks was used to mash the apple mixute in each beaker.
9. The buffer mixture was transferred to the funnel.
10. The amount of juice extracted in each graduated cylinder was logged in table 1.
Results: Simply write what you got for data in your data table. Identify any quantity differences in terms of
percentages/trends. Use in-text citations to cite each
table/graph etc. (e.g., (table 1).) Make sure the citation
is within the sentence.
Conclusion: The results of this experiment
support/do not support (pick one) the original
hypothesis. Give your input about why you think you
got the results you did. Add any other input you have.
State the the scientific significance of the lab. Include
shortcomings of the experiment (what you would do
differently next time). End with suggesting a further study.
Objective: To determine the best enzyme juice extracting mixture from an apple.
Hypothesis: A mixture of 50% cellulose and 50% pectinase will extract more juice than using either one by itself.
Background Info:
- A catalyst speeds up chemical reaction rates without itself being changed.
- Enzymes are special proteins that work as catalysts which promote chemical reactions in living things.
- Enzymes work by either making molecules or breaking them.
- Enzymes usually end with the root ase, such as in pectinase and cellulase.
- Plant support (to stand upright) is due to cellulose in plant cell walls and pectin found in plant cell walls and between
cells in the Middle lamella.
- Cellulase breaks down cellulase and pectinase breaks down pectin.
- To make juice is a two step process: 1. peeling to remove peel & seeds, and juicing to extract up to 20% more juice
from pulp.
- These enzymes come from the fungi Aspergillus niger.
Materials:
plastic wrap acetate water apples
beaker thermometer cellulase funnel
weight boat pectinase graduated cylinder wood sticks
Methods: 1. 50g of small pieces of apple was weighed out in a weight boat.
2. The apple pieces were transerred to the buffer (control) beaker.
3. Steps 1 & 2 were repeated for pectinase, cellulase, and the "both" beakers.
4. 5mL of acetate buffer was measured and added to the buffer (control beaker).
5. The cylinder was rinsed out and step 4 was repeated for the pectinase and cellulase enzyme samples.
6. The pectinase/cellulase sample was added to the "both" beaker.
7. The beakers were covered with plastic wrap and set in a 45°C bath water for 20 min.
8. Separate wooden sticks was used to mash the apple mixute in each beaker.
9. The buffer mixture was transferred to the funnel.
10. The amount of juice extracted in each graduated cylinder was logged in table 1.
Results: Simply write what you got for data in your data table. Identify any quantity differences in terms of
percentages/trends. Use in-text citations to cite each
table/graph etc. (e.g., (table 1).) Make sure the citation
is within the sentence.
Conclusion: The results of this experiment
support/do not support (pick one) the original
hypothesis. Give your input about why you think you
got the results you did. Add any other input you have.
State the the scientific significance of the lab. Include
shortcomings of the experiment (what you would do
differently next time). End with suggesting a further study.
[ eniche.net ]
[ Biology 2009 ]
| Amount of Juice Produced |
| Time (min.) | Buffer | Pectinase | Cellulase | Both |
| 5 | ||||
| 10 | ||||
| 15 |
Table 1. The apple juice extracted from each mixture
