icroscope Lab
Partner:
Objective: To learn the names of the parts of a microscope
To learn the proper use of a microscope
To learn how to prepare objects for viewing under a microscope.
To examine several objects under a microscope using proper techniques.
To determine how the lens system of a microscope changes the position of an object being viewed.
Hypothesis: N/A
Background Info:
- A microscop is a scientific tool that is used to see very small things.
- Things that you cannot see with your eyes alone can be seen using a microscope.
Materials:
pipet swatches of material head and arm hair
magazine scissors water & stain
light microscope slide and coverslip cork
Methods: 1. Study the microscope diagram. Identify the parts of your microscope and try to rerite them from recall on the
blank diagram provided (tape this directtly into your lab notebook).
2. Cut out a small letter e from a magazine and place the letter on a microscope slide. Put a drop of water on
the letter and place a coverslip over the water and the letter.
3. Look to see how the letter e is positioned on the slide and draw the letter as you see it without the aid of the
microscope in your lab notebooks.
4. Turn on the microscope light.
5. Click the low power objective lens (shortest if more than one lens is present) into position. The lens should
be directly over the hole in the stage. Bring the lens close to the slide using the coarse adjustment knob.
NOTE: Be careful not to touch the slide with the lens. This might break the lens and the slide.
6. Look through the eyepiece of the microscope. Carefully bring the letter into focus by slowly turning the
coarse adjustment knob. If you cannot see the letter, move the slide a little bit to be sure the letter is under the
lens.
7. Click the high power objective lens into place. If your microscope has a high power objective, it will also
have a fine adjustment knob. Look through the eyepiece again. Carefully bring the letter e into focus by
slowly turning the fine adjustment knob. NOTE: Never turn the coarse adjustment knob when the high power
objective lens is in place.
8. When the letter e is clearly visible, draw in the space below the position of the letter as you see it through the
microscope. Next, move the slide to the left as you look through the eyepiece.
**Note which way the letter appears to move in your lab notebook.
**Move the slide forward. Note which way it appears to move now.
9. Remove the slide and clean it.
Procedure - cork:
Results: Simply write what you got for data in your data table. Include the number of isopods in each chamber during each
interval. Note any quantity differences in terms of percentages/trends in behavior(s). Use in-text citations to cite each
table/graph etc. (e.g., (table 1).) Make sure the citation is within the sentence.
Objective: To learn the names of the parts of a microscope
To learn the proper use of a microscope
To learn how to prepare objects for viewing under a microscope.
To examine several objects under a microscope using proper techniques.
To determine how the lens system of a microscope changes the position of an object being viewed.
Hypothesis: N/A
Background Info:
- A microscop is a scientific tool that is used to see very small things.
- Things that you cannot see with your eyes alone can be seen using a microscope.
Materials:
pipet swatches of material head and arm hair
magazine scissors water & stain
light microscope slide and coverslip cork
Methods: 1. Study the microscope diagram. Identify the parts of your microscope and try to rerite them from recall on the
blank diagram provided (tape this directtly into your lab notebook).
2. Cut out a small letter e from a magazine and place the letter on a microscope slide. Put a drop of water on
the letter and place a coverslip over the water and the letter.
3. Look to see how the letter e is positioned on the slide and draw the letter as you see it without the aid of the
microscope in your lab notebooks.
4. Turn on the microscope light.
5. Click the low power objective lens (shortest if more than one lens is present) into position. The lens should
be directly over the hole in the stage. Bring the lens close to the slide using the coarse adjustment knob.
NOTE: Be careful not to touch the slide with the lens. This might break the lens and the slide.
6. Look through the eyepiece of the microscope. Carefully bring the letter into focus by slowly turning the
coarse adjustment knob. If you cannot see the letter, move the slide a little bit to be sure the letter is under the
lens.
7. Click the high power objective lens into place. If your microscope has a high power objective, it will also
have a fine adjustment knob. Look through the eyepiece again. Carefully bring the letter e into focus by
slowly turning the fine adjustment knob. NOTE: Never turn the coarse adjustment knob when the high power
objective lens is in place.
8. When the letter e is clearly visible, draw in the space below the position of the letter as you see it through the
microscope. Next, move the slide to the left as you look through the eyepiece.
**Note which way the letter appears to move in your lab notebook.
**Move the slide forward. Note which way it appears to move now.
9. Remove the slide and clean it.
Procedure - cork:
Results: Simply write what you got for data in your data table. Include the number of isopods in each chamber during each
interval. Note any quantity differences in terms of percentages/trends in behavior(s). Use in-text citations to cite each
table/graph etc. (e.g., (table 1).) Make sure the citation is within the sentence.
[ eniche.net ]
Conclusion: The results of this experiment support/do not support (pick one) the original hypothesis. Give your input
about why you think you got the results you did. Add any other input you have. State the the scientific significance of the lab.
Include shortcomings of the experiment (what you would do differently next time). End with suggesting a further study.
about why you think you got the results you did. Add any other input you have. State the the scientific significance of the lab.
Include shortcomings of the experiment (what you would do differently next time). End with suggesting a further study.
